HMGN1
Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Cheval, Cobaye, Lapin
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Immunohistochemistry on Paraffin Sections (20 μg/mL). Immunofluorescence. Western Blot(1/500-1/2000). ELISA (1/5000-1/25000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.
Antigène
HMGN1
(High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
anticorps HMG14, anticorps hmg14, anticorps hmgn1, anticorps hmgn1b, anticorps HMG-14, anticorps Hmg14, anticorps high mobility group nucleosome binding domain 1, anticorps high mobility group nucleosome binding domain 1 S homeolog, anticorps high mobility group nucleosomal binding domain 1, anticorps non-histone chromosomal protein HMG-14, anticorps HMGN1, anticorps hmgn1.S, anticorps hmgn1, anticorps Hmgn1, anticorps LOC785479
Sujet
HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box," that of the HMGN family, the "nucleosomal binding domain," and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."Synonyms: HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1, Non-histone chromosomal protein HMG-14