JNK anticorps (Middle Region)

N° du produit ABIN3042709

Cet anticorps Lapin Polyclonal détecte spécifiquement JNK dans WB et IHC. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 5+ publications.

Aperçu rapide pour JNK anticorps (Middle Region) (ABIN3042709)

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp JNK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-JNK anticorps

Épitope: AA 344-363, Middle Region

Fonction: Anti-MAPK8/9 Antibody Picoband®

Séquence: EREHTIEEWK ELIYKEVMDL

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-MAPK8/9 Antibody (ABIN3042709). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human MAPK8/9, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse -
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Derijard B, Hibi M, Wu IH, Barrett T, Su B, Deng T, Karin M, Davis RJ (Apr 1994). "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain". Cell 76 (6): 1025-37. doi:10.1016/0092-8674(94)90380-8. PMID 8137421. 2. Gupta S, Barrett T, Whitmarsh AJ, Cavanagh J, Sluss HK, Derijard B, Davis RJ (Jul 1996). "Selective interaction of JNK protein kinase isoforms with transcription factors". EMBO J 15 (11): 2760-70. PMC 450211. PMID 8654373.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-JNK (ABIN3042709)

Shangguan, Jiang, Pan, Xiao, Tan, Tie, Qin, Deng, Chen, Wang: "Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats." dans: Cell death & disease, Vol. 8, Issue 10, pp. e3157, (2018) (PubMed).

Wang, Gao, Wu, Wang, Song, Chen, Zhang, Zhang, Zhang, Yu: "Pseudolaric acid B induced autophagy, but not apoptosis, in MRC5 human fibroblast cells." dans: Oncology letters, Vol. 15, Issue 1, pp. 863-870, (2018) (PubMed).

Liu, Lv, Ning, Yang, Zhu: "Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model." dans: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2284-2292, (2016) (PubMed).

Bu, Zhao, Zhang, Wang, Li, Yan: "Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress." dans: American journal of cancer research, Vol. 6, Issue 5, pp. 924-36, (2016) (PubMed).

Ding, Zou, Li, Tian, Abdelalim, Du, She, Wang, Tan, Wang, Chen, Lv, Chang: "Study of histopathological and molecular changes of rat kidney under simulated weightlessness and resistance training protective effect." dans: PLoS ONE, Vol. 6, Issue 5, pp. e20008, (2011) (PubMed).

Détails sur JNK

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Autre désignation: MAPK8

Sujet:

Synonyms: Mitogen-activated protein kinase 8,MAP kinase 8,MAPK 8,2.7.11.24,JNK-46,Stress-activated protein kinase 1c,SAPK1c,Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1,MAPK8,JNK1, PRKM8, SAPK1, SAPK1C, JNK2, MAPK9

Background: Mitogen-activated protein kinase 8 is an enzyme that in humans is encoded by the MAPK8 gene. The protein encoded by this gene is a member of the MAP kinase family. The International Radiation Hybrid Mapping Consortium mapped the MAPK8 gene to chromosome 10. The single long open reading frame encodes a putative protein kinase with a predicted mass of 44.2 kD. This kinase play a key role in T cell proliferation, apoptosis and differentiation. The protein encoded by MAPK9 gene is also a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Poids moléculaire: 65 kDa

UniProt: P45983, P45984

Pathways: Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins