ERK1 anticorps (Middle Region)

N° du produit ABIN3044377

Cet anticorps Lapin Polyclonal détecte spécifiquement ERK1 dans WB, IHC et ICC. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 7+ publications.

Aperçu rapide pour ERK1 anticorps (Middle Region) (ABIN3044377)

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Détail du produit anti-ERK1 anticorps

Épitope: AA 287-301, Middle Region

Fonction: Anti-ERK1/MAPK3 Antibody Picoband®

Séquence: KTKVAWAKLF PKSDS

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-ERK1/MAPK3 Antibody (ABIN3044377). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human ERK1, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Garcia F, Zalba G, Paez G, Encio I, de Miguel C (Apr 1999). "Molecular cloning and characterization of the human p44 mitogen-activated protein kinase gene". Genomics 50 (1): 69-78. 2. "Entrez Gene: MAPK3 mitogen-activated protein kinase 3". 3. Charest, D. L., Mordret, G., Harder, K. W., Jirik, F., Pelech, S. L. Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1. Molec. Cell. Biol. 13: 4679-4690, 1993.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-ERK1 (ABIN3044377)

Han, Wu, Chang, Zhu, Xiao, Li, Zhang, Zhang: "Disulfiram inhibits TGF-β-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-κB/Snail pathway." dans: Oncotarget, Vol. 6, Issue 38, pp. 40907-19, (2016) (PubMed).

Tang, Zhang, Qi, Chen, Li, Liu, He, Li, Zu: "Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line." dans: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1851-1856, (2015) (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." dans: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, (2014) (PubMed).

Ma, Wang, Chen, Chen, Wang, Aisa: "Icariin and icaritin stimulate the proliferation of SKBr3 cells through the GPER1-mediated modulation of the EGFR-MAPK signaling pathway." dans: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1627-34, (2014) (PubMed).

Yang, Han, Liu, Hu, Li, Zhu: "Negative pressure wound therapy is associated with up-regulation of bFGF and ERK1/2 in human diabetic foot wounds." dans: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 22, Issue 4, pp. 548-54, (2014) (PubMed).

Li, Zhu, Liu, Liu, Wang, Xiong, Shen, Hu, Zheng: "ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299." dans: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2460-7, (2013) (PubMed).

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." dans: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, (2007) (PubMed).

Détails sur ERK1

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Autre désignation: MAPK3

Sujet:

Synonyms: Mitogen-activated protein kinase 3,MAP kinase 3,MAPK 3,2.7.11.24,ERT2,Extracellular signal-regulated kinase 1,ERK-1,Insulin-stimulated MAP2 kinase,MAP kinase isoform p44,p44-MAPK,Microtubule-associated protein 2 kinase,p44-ERK1,MAPK3,ERK1, PRKM3,

Background: Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Poids moléculaire: 100 kDa

UniProt: P27361

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins