p53 anticorps (AA 371-380)

N° du produit ABIN306866

L’anticorps Souris Monoclonal anti-p53 a été validé pour WB, IHC, ELISA et IP. Il convient pour détecter p53 dans des échantillons de Humain.

Aperçu rapide pour p53 anticorps (AA 371-380) (ABIN306866)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: HR231

Détail du produit anti-p53 anticorps

Épitope: AA 371-380

Réactivité croisée: Humain, Singe, Souris, Rat

Attributs du produit: p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from immunized with full length human p53 protein and mouse myeloma cells.

Isotype: IgG2b

Information d'application

Indications d'application: This antibody can be used for Western blotting (1-5 μg/mL), immunoprecipitation, ELISA and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

UniProt: Q9BRQ8

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg