p53 anticorps (full length)

N° du produit ABIN306868

Cet anticorps Souris Monoclonal détecte spécifiquement p53 dans WB, IHC (p) et IP. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour p53 anticorps (full length) (ABIN306868)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: BP53-12

Détail du produit anti-p53 anticorps

Épitope: AA 20-25, full length

Fonction: Mouse anti Human only p53 (a.a. 20-25)

Réactivité croisée: Humain

Attributs du produit: p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from immunized with full length wild-type human p53 protein and mouse myeloma cells.

Isotype: IgG2a

Information d'application

Indications d'application: This antibody can be used for Western blotting (0.5-1 μg/mL), immunohistochemistry on formalin/paraffain embedded tissues (1-2 μg/mL) and immunoprecipitation (2 μg/mg of protein lysate). Antibody reacts with mutant as well as wild-type p53 protein. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg