MUC1 anticorps
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- Antigène Voir toutes MUC1 Anticorps
- MUC1 (Mucin 1 (MUC1))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MUC1 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Réactivité croisée
- Humain
- Attributs du produit
- Episialin,Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90 % breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed, is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400 kDa) and a smaller subunit (14-28 kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells, it can bind -catenin, GSK3 and it associates with Grb2-SOS upon phosphorylation.
- Purification
- Protein A/G Chromatography
- Immunogène
- Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a Muc1 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH and mouse myeloma SP2 cells.
- Clone
- VU-4H5
- Isotype
- IgG1
- Top Product
- Discover our top product MUC1 Anticorps primaire
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- Indications d'application
- Antibody can be used for immunohistochemistry on frozen and paraffin embedded tissues, ELISA and Western blotting. Optimal concentration should be evaluated by serial dilutions. Antibody can only detect Muc1 if the threonine residue of the PDTRPAP of the tandem repeat region is non-glycosylated4.
- Restrictions
- For Research Use only
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- Buffer
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- Antigène
- MUC1 (Mucin 1 (MUC1))
- Autre désignation
- Muc1 (MUC1 Produits)
- Synonymes
- anticorps CA 15-3, anticorps CD227, anticorps EMA, anticorps H23AG, anticorps KL-6, anticorps MAM6, anticorps MCKD1, anticorps MUC-1, anticorps MUC-1/SEC, anticorps MUC-1/X, anticorps MUC1/ZD, anticorps PEM, anticorps PEMT, anticorps PUM, anticorps Muc-1, anticorps mucin 1, cell surface associated, anticorps mucin 1, transmembrane, anticorps MUC1, anticorps Muc1
- UniProt
- P15941
- Pathways
- Negative Regulation of intrinsic apoptotic Signaling
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