COL18A1 anticorps

N° du produit ABIN307188

Détail du produit anti-COL18A1 anticorps

Antigène: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp COL18A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.

Purification: Thiophilic adsorption

Immunogène: Full-length recombinant murine endostatin.

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (1:100), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50 ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 8.0, 0.05 % sodium azide, before the addition of glycerol to 30 %. Sourced in Ascites.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur COL18A1

Antigène: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Autre désignation: COL18A1 / Endostatin (COL18A1 Produits)

Synonymes: anticorps KNO, anticorps KNO1, anticorps KS, anticorps cb371, anticorps cb529, anticorps fc96d06, anticorps sb:cb529, anticorps wu:fb47h10, anticorps wu:fb77g12, anticorps wu:fc50f04, anticorps wu:fc96d06, anticorps wu:fk02h12, anticorps kno, anticorps kno1, anticorps collagen type XVIII alpha 1 chain, anticorps collagen type XVIII alpha 1 chain a, anticorps collagen, type XVIII, alpha 1, anticorps collagen type XVIII alpha 1, anticorps COL18A1, anticorps col18a1a, anticorps Col18a1, anticorps col18a1

Classe de substances: Chemical

Sujet: Name/Gene ID: COL18A1
Family: Collagen

Synonyms: COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1(XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1(xviii), Knobloch syndrome, type 1

ID gène: 80781

UniProt: P39060