MYL2 anticorps (pSer18)

N° du produit ABIN319224

Détail du produit anti-MYL2 anticorps

Antigène: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Épitope: pSer18

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYL2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of Myosin Light Chain 2 only when phosphorylated at Serine 18.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from Human Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-Sp-N-V).

Information d'application

Indications d'application: Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide, 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C.

Détails sur MYL2

Antigène: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Autre désignation: Myosin Light Chain 2 (MYL2) (MYL2 Produits)

Synonymes: anticorps CMH10, anticorps MLC2, anticorps 2410014J02Rik, anticorps MLC-2, anticorps Mlc2, anticorps MLC-2v, anticorps Mlc2v, anticorps Mylpc, anticorps Mlc-2, anticorps myl2, anticorps zgc:136848, anticorps XMLC2, anticorps cmh10, anticorps mlc2, anticorps G2, anticorps MLC-2A, anticorps MLC2V, anticorps myosin light chain 2, anticorps myosin light chain, phosphorylatable, fast skeletal muscle, anticorps myosin, light polypeptide 2, regulatory, cardiac, slow, anticorps myosin, light chain 2b, regulatory, cardiac, slow, anticorps myosin light chain 2 S homeolog, anticorps myosin, light chain 2, regulatory, cardiac, slow, anticorps MYL2, anticorps Mylpf, anticorps Myl2, anticorps myl2b, anticorps myl2.S

Sujet: Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.Synonyms: MLC-2, MLC-2v, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform

Poids moléculaire: 18 kDa

ID gène: 4633

NCBI Accession: NP_000423

UniProt: P10916

Pathways: Myometrial Relaxation and Contraction