p53 anticorps (pSer46)

N° du produit ABIN3201005

Cet anticorps anti-p53 est un anticorps Souris Monoclonal détectant p53 dans WB, ELISA et IHC (p). Adapté pour Humain. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour p53 anticorps (pSer46) (ABIN3201005)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 36

Détail du produit anti-p53 anticorps

Épitope: pSer46

Réactivité croisée (Details): Not tested in other species.

Purification: Purified from serum-free culture medium of hybridoma (#36) by proprietary chromatography procedures under mild conditions.

Stérilité: Sterile filtered

Immunogène: Synthetic peptide containing pSer46 of p53

Isotype: IgG1 kappa

Information d'application

Indications d'application: 1) Western blotting: 1,000~2,000 dilution
2) Immunohistochemistry (assay dependent)
3) ELISA
Other applications have not been tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Upon arrival centrifuge briefly and store at -20 C.

Références pour anticorps anti-p53 (ABIN3201005)

Taira, Yamamoto, Yamaguchi, Miki, Yoshida: "ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage." dans: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4909-19, (2010) (PubMed).

Kodama, Otsubo, Hirota, Yokota, Enari, Taya: "Requirement of ATM for rapid p53 phosphorylation at Ser46 without Ser/Thr-Gln sequences." dans: Molecular and cellular biology, Vol. 30, Issue 7, pp. 1620-33, (2010) (PubMed).

Taira, Nihira, Yamaguchi, Miki, Yoshida: "DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage." dans: Molecular cell, Vol. 25, Issue 5, pp. 725-38, (2007) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI As to the kinase of phosphorylation of Ser46, involvement of DYRK2 and ATM have been implicated.

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg