p53 anticorps (pSer315)

N° du produit ABIN3201006

L’anticorps Souris Monoclonal anti-p53 a été validé pour WB, ELISA et IHC (p). Il convient pour détecter p53 dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour p53 anticorps (pSer315) (ABIN3201006)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 18

Détail du produit anti-p53 anticorps

Épitope: pSer315

Attributs du produit: This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone #18) which produces monoclonal antibody that specifically recognizes human p53 protein with phospholyrated Ser315.

Purification: Purified

Immunogène: Synthetic peptide containing pSer315 of p53

Isotype: IgG2b kappa

Information d'application

Indications d'application: 1. Western blotting: 1,000~2000 dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Upon arrival centrifuge briefly and store at -20 C.

Références pour anticorps anti-p53 (ABIN3201006)

Bode, Dong: "Post-translational modification of p53 in tumorigenesis." dans: Nature reviews. Cancer, Vol. 4, Issue 10, pp. 793-805, (2004) (PubMed).

Katayama, Sasai, Kawai, Yuan, Bondaruk, Suzuki, Fujii, Arlinghaus, Czerniak, Sen: "Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and inhibition of p53." dans: Nature genetics, Vol. 36, Issue 1, pp. 55-62, (2003) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Ser315 is phosphorylated by aurora kinase and cycline-dependent kinases when cells are subjected to stress such as DNA damage and microtubule disruption by nocodazole. However the effect of the phosphorylation on the function of p53 is mostly unknown.

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg