p53 anticorps (acLys315)

N° du produit ABIN3201007

L’anticorps Souris Monoclonal anti-p53 a été validé pour WB, ELISA, FACS et IP. Il convient pour détecter p53 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour p53 anticorps (acLys315) (ABIN3201007)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 10E5

Détail du produit anti-p53 anticorps

Épitope: acLys315

Attributs du produit: This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone 10E5), which produces monoclonal antibody that specifically recognizes human p53 protein with acetylated Lys120.

Purification: Purified

Immunogène: synthetic peptide containing acLys315

Isotype: IgG1 kappa

Information d'application

Indications d'application: 1. Western blotting: ~1 g/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Upon arrival centrifuge briefly and store at -20 C.

Références pour anticorps anti-p53 (ABIN3201007)

Tyteca, Legube, Trouche: "To die or not to die: a HAT trick." dans: Molecular cell, Vol. 24, Issue 6, pp. 807-8, (2006) (PubMed).

Tang, Luo, Zhang, Gu: "Tip60-dependent acetylation of p53 modulates the decision between cell-cycle arrest and apoptosis." dans: Molecular cell, Vol. 24, Issue 6, pp. 827-39, (2006) (PubMed).

Sykes, Mellert, Holbert, Li, Marmorstein, Lane, McMahon: "Acetylation of the p53 DNA-binding domain regulates apoptosis induction." dans: Molecular cell, Vol. 24, Issue 6, pp. 841-51, (2006) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60, and activates transcription of proapoptotic genes, BAX and PUMA.

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg