CHEK1 anticorps (Checkpoint Kinase 1)

Details for Product anti-CHEK1 Antibody No. ABIN3209997
Antigène
Reactivité
Humain, Souris
542
207
180
14
13
9
9
8
7
5
5
3
1
1
1
1
Hôte
Souris
494
44
10
7
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp CHEK1 est non-conjugé
23
14
12
12
12
12
12
12
12
11
10
8
3
3
3
3
3
2
1
Application
ELISA, Western Blotting (WB)
420
215
166
74
67
40
25
21
20
14
14
6
6
3
2
Options
Immunogène CHK1 antibody was raised in Mouse using a purified recombinant fragment of CHK1 expressed in E. coli as the immunogen.
Clone 2G1D5
Isotype IgG1
 Réactivité croisée Souris
Purification Purified antibody.
Antigène
Autre désignation CHK1 (CHEK1 Antibody Extrait)
Sujet CHK1 is a kinase that phosphorylates Cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Synonyms: Monoclonal CHK1 antibody, Anti-CHK1 antibody, CHK-1 antibody, CHK 1 antibody, CHEK1 antibody.
Pathways Signalisation p53, Apoptose, Cycle Cellulaire
Indications d'application ELISA: 1:10,000, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator.
Restrictions For Research Use only
Concentration Lot specific
Buffer Purified antibody supplied in PBS containing 0.03 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Stock RT/-20 °C
Stockage commentaire Store at 4 °C for short term storage. Store at -20 °C for long term storage.
Images (Fournisseur)
Image no. 1 for anti-Checkpoint Kinase 1 (CHEK1) antibody (ABIN3209997) Western Blot showing using CHK1 antibody used against A431 (1), Hela (2), NIH/3T3 (3)...
Image no. 2 for anti-Checkpoint Kinase 1 (CHEK1) antibody (ABIN3209997) Western Blot showing CHK1 antibody used against HEK293T cells transfected with the pC...