GTPase NRas anticorps (AA 167-179)

N° du produit ABIN337227

Cet anticorps anti-GTPase NRas Polyclonal Chèvre (ABIN337227) détecte spécifiquement GTPase NRas dans WB, ELISA, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Chien, Boeuf (Vache), Cheval, Roussette (Chauve-souris) et Porc.

Aperçu rapide pour GTPase NRas anticorps (AA 167-179) (ABIN337227)

Antigène: GTPase NRas (NRAS)

Reactivité: Humain, Souris, Chien, Boeuf (Vache), Cheval, Roussette (Chauve-souris), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GTPase NRas est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GTPase NRas anticorps

Épitope: AA 167-179

Specificité: Human NRAS.

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%) Marmoset, Rat, Hamster, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%), Marmoset, Rat, Hamster, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GTPase NRas

Antigène: GTPase NRas (NRAS)

Autre désignation: NRAS / N-ras

Sujet: Name/Gene ID: NRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: NRAS, HRAS1, NRAS1, NS6, ALPS4, Transforming protein N-Ras, N-ras, N-ras protein part 4, GTPase NRas

ID gène: 4893

NCBI Accession: NP_002515

UniProt: P01111

Pathways: Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling