Erythrocyte Ankyrin anticorps (AA 1971-1983)

N° du produit ABIN343025

Détail du produit anti-Erythrocyte Ankyrin anticorps

Antigène: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Épitope: AA 1971-1983

Reactivité: Humain, Souris, Rat, Chien, Lapin, Cheval, Boeuf (Vache), Singe, Roussette (Chauve-souris), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Erythrocyte Ankyrin est non-conjugé

Application: ELISA

Specificité: Human ANK1. This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%) Turkey (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-QIVKRASLKRGKQ from the C-terminus of human ANK1 (NP_000028.3, NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%), Turkey (92%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave approx. 19-23 kD bands in Human Skeletal Muscle lysates after 0.01 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kD bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kD according to NP_065211.2. The 19-22 kD bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Erythrocyte Ankyrin

Antigène: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Autre désignation: ANK1 / Ankyrin (ANK1 Produits)

Synonymes: anticorps CHANK1, anticorps wu:fa09h06, anticorps zgc:101835, anticorps ank, anticorps sph1, anticorps sph2, anticorps ANK1, anticorps ANK, anticorps SPH1, anticorps SPH2, anticorps Ank-1, anticorps nb, anticorps pale, anticorps ankyrin 1, anticorps ankyrin 1, erythrocytic a, anticorps ankyrin 1 L homeolog, anticorps ankyrin 1, erythrocytic, anticorps ankyrin 1, erythroid, anticorps ANK1, anticorps ank1a, anticorps ank1, anticorps ank1.L, anticorps Ank1

Sujet: Name/Gene ID: ANK1

Synonyms: ANK1, ANK-1, Ankyrin-1, Ankyrin-R, Erythrocyte ankyrin, ANK, Ankyrin, Ankyrin 1, erythrocytic, SPH2, SPH1

ID gène: 286

NCBI Accession: NP_000028, NP_065208, NP_065209, NP_065210, NP_065211, NP_065213

UniProt: P16157

Pathways: Synaptic Membrane