SMAD1 anticorps (Internal Region)

N° du produit ABIN964911

Détail du produit anti-SMAD1 anticorps

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD1 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This affinity-purified antibody is directed against the phosphorylated form of human SMAD1 protein at the pS206 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human SMAD1 pS206 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human SMAD1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with SMAD1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Réactivité croisée: Souris, Rat (Rattus), Chien

Attributs du produit: SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to phosphorylated SMAD1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This antibody is specific for the phosphorylated pS206 of SMAD1. Stimulation of EGF, TGFbeta BMP2, and 0.5M NaCl are recommended for 1 hour.

Commentaires:

Gene Name: SMAD1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.31 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Date de péremption: 3 months

Détails sur SMAD1

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Autre désignation: SMAD1 (SMAD1 Produits)

Synonymes: anticorps MLP, anticorps Xmad1, anticorps Xsmad1, anticorps bsp1, anticorps jv4-1, anticorps jv41, anticorps madh1, anticorps madr1, anticorps smad-1, anticorps MADH1, anticorps fb39h09, anticorps wu:fb39h09, anticorps wu:fb97e04, anticorps 2/23, anticorps CG12399, anticorps Dmel\\CG12399, anticorps E(zen)2, anticorps En(vvl), anticorps MAD, anticorps Mat, anticorps P-Mad, anticorps P-mad, anticorps PMad, anticorps Smad, anticorps Smad1, anticorps apg, anticorps c28, anticorps dMAD, anticorps dMad, anticorps l(2)K00237, anticorps l(2)k00237, anticorps mad, anticorps p-Mad, anticorps pMAD, anticorps pMad, anticorps pSmad, anticorps BSP-1, anticorps BSP1, anticorps JV4-1, anticorps JV41, anticorps MADR1, anticorps AI528653, anticorps Madh1, anticorps Madr1, anticorps Mlp1, anticorps MusMLP, anticorps smad1, anticorps xmad, anticorps SMAD family member 1, anticorps Mothers against dpp, anticorps SMAD family member 1 L homeolog, anticorps smad1, anticorps SMAD1, anticorps Mad, anticorps Smad1, anticorps smad1.L

Sujet: SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro; this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.
Synonyms: Mothers against DPP homolog 1 Mad-related protein 1 SMAD 1 hSMAD1 Transforming growth factor-beta-signaling protein 1 BSP-1 JV4-1

ID gène: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development