CHEK1 anticorps

N° du produit ABIN356395

Cet anticorps Souris Monoclonal détecte spécifiquement CHEK1 dans WB et EIA. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour CHEK1 anticorps (ABIN356395)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CHEK1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 2G1D5

Détail du produit anti-CHEK1 anticorps

Specificité: This antibody is specific to ChK1.

Attributs du produit: Synonyms: CHEK1, CHEK-1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This monoclonal antibody is generated from mice immunized with Ni-NTA purifiedrecombinant protein CHK1 expressed in E. Coli strain M15.

Information d'application

Indications d'application: ELISA: 1/10,000. Western blotting: 1/1,000-1/4,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHK1

Sujet: Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The protein exhibits altered mobility in immunoblotting when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

ID gène: 1111

UniProt: O14757

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN