CHEK1 anticorps

N° du produit ABIN357628

Cet anticorps Lapin Polyclonal détecte spécifiquement CHEK1 dans WB et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour CHEK1 anticorps (ABIN357628)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-CHEK1 anticorps

Specificité: This antibody is specific to CHK1.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography followed by peptide affinity purification.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S280 of human CHK1.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHK1

Sujet: Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Poids moléculaire: 67612 Da

ID gène: 1111, 5874

UniProt: O14757

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN