PRKAR1B anticorps (N-Term)

N° du produit ABIN359120

Détail du produit anti-PRKAR1B anticorps

Antigène: PRKAR1B (Protein Kinase, CAMP-Dependent, Regulatory, Type I, beta (PRKAR1B))

Épitope: N-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAR1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody reacts to PKA R1 beta (PRKAR1B).

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PRKAR1B.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur PRKAR1B

Antigène: PRKAR1B (Protein Kinase, CAMP-Dependent, Regulatory, Type I, beta (PRKAR1B))

Autre désignation: PRKAR1B (PRKAR1B Produits)

Synonymes: anticorps PRKAR1B, anticorps AI385716, anticorps RIbeta, anticorps RGD1563094, anticorps PRKAR1, anticorps zgc:153624, anticorps prkar1, anticorps protein kinase cAMP-dependent type I regulatory subunit beta, anticorps protein kinase, cAMP dependent regulatory, type I beta, anticorps protein kinase cAMP-dependent type 1 regulatory subunit beta, anticorps protein kinase, cAMP-dependent, regulatory, type I, beta, anticorps protein kinase, cAMP-dependent, regulatory subunit type I beta S homeolog, anticorps PRKAR1B, anticorps prkar1b, anticorps Prkar1b, anticorps prkar1b.S

Sujet: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93 % amino acid sequence similarity), whereas Cγ displays 83 % and 79 % similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.Synonyms: PKA regulatory subunit I beta, PRKAR1, cAMP-dependent protein kinase type I-beta regulatory subunit

ID gène: 5575, 9606

UniProt: P31321

Pathways: Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma