Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificité
The antibody detects endogenous level of G3BP-1 only when phosphorylated at serine 232.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pSer232 (S-S-S (p) -P-A) derived from Human G3BP-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.