ATM anticorps (pSer1981)

N° du produit ABIN362099

Il existe 4+ publications pour ce produit. L’anticorps anti-ATM Polyclonal Lapin est utilisé pour la détection de ATM dans des échantillons de Humain et Souris. Il a été validé pour IHC.

Aperçu rapide pour ATM anticorps (pSer1981) (ABIN362099)

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATM est non-conjugé

Application: Immunohistochemistry (IHC)

Détail du produit anti-ATM anticorps

Épitope: pSer1981

Specificité: The antibody detects endogenous level of ATM only when phosphorylated at serine 1981.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer1981 (E-G-S (p) -Q-S) derived from Human ATM. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100  

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-ATM (ABIN362099)

Inaba, Kuboniwa, Sugita, Lamont, Amano: "Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts." dans: Infection and immunity, Vol. 80, Issue 8, pp. 2847-57, (2012) (PubMed).

Mahalingam, Tay, Tan, Chai, Wang: "Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2-ATM pathway in telomerase-overexpressing primary fibroblasts." dans: The FEBS journal, Vol. 278, Issue 19, pp. 3724-38, (2011) (PubMed).

Leemput, Masson, Bigot, Errachid, Dansault, Provost, Gadin, Aoufouchi, Menasche, Abitbol: "ATM localization and gene expression in the adult mouse eye." dans: Molecular vision, Vol. 15, pp. 393-416, (2009) (PubMed).

Kang, Guo, Tan, Zhao, Tang, Lu: "Expression status of ataxia-telangiectasia-mutated gene correlated with prognosis in advanced gastric cancer." dans: Mutation research, Vol. 638, Issue 1-2, pp. 17-25, (2008) (PubMed).

Détails sur ATM

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Autre désignation: ATM

Sujet: ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates, thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 350 kDa

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus