Src anticorps (pTyr529)

N° du produit ABIN362154

Il existe 1 publication pour ce produit. L’anticorps anti-Src Polyclonal Lapin est utilisé pour la détection de Src dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour Src anticorps (pTyr529) (ABIN362154)

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Src est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-Src anticorps

Épitope: pTyr529

Specificité: The antibody detects endogenous level of Src only when phosphorylated at tyrosine 529.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pTyr529 (P-Q-Y (p) -Q-P) derived from Human Src. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-Src (ABIN362154)

Zheng, Xia, Hawke, Halle, Tremblay, Gao, Zhou, Aldape, Cobb, Xie, He, Lu: "FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST." dans: Molecular cell, Vol. 35, Issue 1, pp. 11-25, (2009) (PubMed).

Détails sur Src

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Autre désignation: Src

Sujet: Proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme, phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Poids moléculaire: 60 kDa

NCBI Accession: NP_005408

UniProt: P12931

Pathways: Signalistation JAK/STAT, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling