p53 anticorps (AA 44-48)

N° du produit ABIN362654

Cet anticorps Lapin Polyclonal détecte spécifiquement p53 dans WB. Il présente une réactivité avec des échantillons de Humain.Il a été cité dans 1 publication.

Aperçu rapide pour p53 anticorps (AA 44-48) (ABIN362654)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-p53 anticorps

Épitope: AA 44-48

Specificité: The antibody detects endogenous level of total p53 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Peptide sequence around AA 44-48 (M-L-S-P-D) derived from Human p53. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-p53 (ABIN362654)

Zhang, Ruan, Yan, Li, Wu, Tao, Zhang, Zheng, Wang, Lu: "Xanthatin induces cell cycle arrest at G2/M checkpoint and apoptosis via disrupting NF-?B pathway in A549 non-small-cell lung cancer cells." dans: Molecules (Basel, Switzerland), Vol. 17, Issue 4, pp. 3736-50, (2012) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Di Stefano V, et al. (2005) Oncogene. 24(35):5431-5442. Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959. Wang L, et al. (2005) Oncogene. 24(18): 3020-3027.

Poids moléculaire: 53 kDa

NCBI Accession: NP_000537

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg