APOB anticorps (AA 3984-3995)

N° du produit ABIN364413

Détail du produit anti-APOB anticorps

Antigène: APOB (Apolipoprotein B (APOB))

Épitope: AA 3984-3995

Reactivité: Humain, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APOB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human APOB.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-TDLHLRYQKDKK from an internal region of human APOB (NP_000375.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Band corresponding to the expected size of ApoB100 is seen along with additional bands at lower molecular weights.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur APOB

Antigène: APOB (Apolipoprotein B (APOB))

Autre désignation: Apolipoprotein B / APOB (APOB Produits)

Synonymes: anticorps FLDB, anticorps LDLCQ4, anticorps Ac1-060, anticorps Apo B-100, anticorps ApoB-100, anticorps ApoB-48, anticorps AI315052, anticorps apob-100, anticorps apob-48, anticorps APOB-100, anticorps ApoB(100), anticorps APOB, anticorps apolipoprotein B, anticorps APOB, anticorps Apob

Sujet: Name/Gene ID: APOB

Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48

ID gène: 338

NCBI Accession: NP_000375

UniProt: P04114

Pathways: Lipid Metabolism