Annexin a1 anticorps (AA 324-337)

N° du produit ABIN364419

Détail du produit anti-Annexin a1 anticorps

Antigène: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Épitope: AA 324-337

Reactivité: Humain, Souris, Rat, Chien, Cobaye, Porc, Lapin, Singe, Boeuf (Vache), Cheval, Roussette (Chauve-souris), Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Annexin a1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human ANXA1 / Annexin A1.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig (100%) Platypus (93%) Lizard (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide CQAILDETKGDYEK from the C-terminus of human ANXA1 / Annexin A1 (NP_000691.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig (100%), Platypus (93%), Lizard (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 1.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, Cancer Res. 2000 Nov15,60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Annexin a1

Antigène: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Autre désignation: ANXA1 / Annexin A1 (ANXA1 Produits)

Synonymes: anticorps anx1a, anticorps anxa1, anticorps wu:fa01d11, anticorps wu:fa05d11, anticorps wu:fk69h01, anticorps MGC64363, anticorps anx1, anticorps lpc1, anticorps MGC89164, anticorps ANXA1, anticorps ANX1, anticorps LPC1, anticorps p35, anticorps Anx-1, anticorps Anx-A1, anticorps C430014K04Rik, anticorps Lpc-1, anticorps Lpc1, anticorps Anx1, anticorps annexin A1a, anticorps annexin A1, anticorps annexin A1 L homeolog, anticorps Annexin A1, anticorps anxa1a, anticorps anxa1, anticorps anxa1.L, anticorps ANXA1, anticorps LOC100335033, anticorps Anxa1

Sujet: Name/Gene ID: ANXA1

Synonyms: ANXA1, Annexin A1, Annexin I, Annexin-1, ANX1, Calpactin II, Chromobindin-9, Lipocortin I, Annexin I (lipocortin I), Calpactin-2, LPC1

ID gène: 301

NCBI Accession: NP_000691

UniProt: P04083

Pathways: Hormone Transport