CD25 anticorps

N° du produit ABIN370883

Détail du produit anti-CD25 anticorps

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificité: This antibody is specific to Low affinity alpha chain IL-2 Receptor (IL-2Ra), Mr 55 kDa. The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7. While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antobody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone. (Ref.3,8,9)

Attributs du produit: Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen

Purification: Purified

Immunogène: IL-2-dependent BALB/c mouse helper T-cell clone HT-2. (Ref. 1)

Clone: 7D4

Isotype: IgM

Information d'application

Indications d'application: Flow Cytometry. (Ref.1,3,7,8)Immunoprecipitation. (Ref.1,3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25 / IL2RA (IL2RA Produits)

Synonymes: anticorps CD25, anticorps IDDM10, anticorps IL2R, anticorps TCGFR, anticorps IL2RAC, anticorps IL-2RA, anticorps Il2r, anticorps Ly-43, anticorps interleukin 2 receptor subunit alpha, anticorps interleukin-2 receptor subunit alpha-like, anticorps interleukin 2 receptor, alpha chain, anticorps IL2RA, anticorps Il2ra, anticorps LOC100222957

Sujet: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. (Ref.2) CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. (Ref.1,3,4) It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. (Ref.5,6) CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus. (Ref.6,7)Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

ID gène: 16184

UniProt: P01590

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation