FYN anticorps (N-Term)

N° du produit ABIN374105

L’anticorps anti-FYN Polyclonal Chèvre est utilisé pour la détection de FYN dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien et Porc. Il a été validé pour WB.

Aperçu rapide pour FYN anticorps (N-Term) (ABIN374105)

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FYN est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-FYN anticorps

Épitope: N-Term

Séquence: VQCKDKEATK LTE

Specificité: This antibody is expected to recognise all three reported isoforms of FYN (NP_002028.1, NP_694592.1 and NP_694593.1).

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from the N Terminus of the protein sequence according to NP_002028.1, NP_694592.1, NP_694593.1. Genename: FYN

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 55-60 kDa band observed in Jurkat, A431 and NIH-3T3lysates (predicted MW of 60 kDa according to NP_002028.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur FYN

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Autre désignation: FYN

Sujet: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn

ID gène: 2534

NCBI Accession: NP_002028

UniProt: P06241

Pathways: Signalistation JAK/STAT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling