NOS1 anticorps (C-Term)
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- Antigène Voir toutes NOS1 Anticorps
- NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
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Épitope
- C-Term
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Reactivité
- Humain, Rat, Souris, Chien, Boeuf (Vache)
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NOS1 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Séquence
- C-ESKKDTDEVF SS
- Specificité
- This antibody detects NOS1 (C-term).
- Réactivité croisée (Details)
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Species reactivity (expected):Rat, Canine (Dog) and Bovine.
Species reactivity (tested):Human, Mouse. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Immunogène
- Peptide from C Terminus of the protein sequence according to NP_000611.1 Genename: NOS1
- Top Product
- Discover our top product NOS1 Anticorps primaire
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- Indications d'application
- Peptide ELISA: Limit Dilution: 1/64000. Western blot: 0.3-1 μg/mL. Approx 160-170 kDa band observed in Human Skeletal Muscleand Mouse Brain lysates (calculated MW of 160 kDa according to NP_000611.1). Immunohistochemistry on Olfactory bulb in mice. Immunofluorescence. (Figure 5. in Ref.1)
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
- Autre désignation
- NOS1 (NOS1 Produits)
- Synonymes
- anticorps NOS1, anticorps IHPS1, anticorps N-NOS, anticorps NC-NOS, anticorps NOS, anticorps bNOS, anticorps nNOS, anticorps 2310005C01Rik, anticorps NO, anticorps NOS-I, anticorps Nos-1, anticorps nos, anticorps ihps1, anticorps nnos, anticorps nos1, anticorps nos1-A, anticorps nanos C2HC-type zinc finger 1, anticorps nitric oxide synthase 1, anticorps nitric oxide synthase 1, neuronal, anticorps nitric oxide synthase 1 (neuronal), anticorps nitric oxide synthase 1 L homeolog, anticorps NANOS1, anticorps NOS1, anticorps Nos1, anticorps nos1, anticorps nos1.L
- Sujet
- Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).Synonyms: Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Nitric Oxide Synthase, bNOS, nNOS
- ID gène
- 4842
- NCBI Accession
- NP_000611
- UniProt
- P29475
- Pathways
- Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction
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