BAK1 anticorps (N-Term)

N° du produit ABIN374174

Il existe 1 publication pour ce produit. L’anticorps anti-BAK1 Polyclonal Chèvre est utilisé pour la détection de BAK1 dans des échantillons de Humain, Souris, Rat et Porc. Il a été validé pour WB et EIA.

Aperçu rapide pour BAK1 anticorps (N-Term) (ABIN374174)

Antigène: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp BAK1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-BAK1 anticorps

Épitope: N-Term

Séquence: ASGQGPGPPR QE-C

Specificité: This antibody detects Bcl-2-like 7 at N-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from N Terminus of the protein sequence according to NP_001179.1 Genename: BAK1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western Blot: 0.5-1 μg/mL. Approx 25 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Références pour anticorps anti-BAK1 (ABIN374174)

Huang, Li, Pang, Chen, Xiang, Feng, Su, Li, Zhang, Liu: "MicroRNA-29c Correlates with Neuroprotection Induced by FNS by Targeting Both Birc2 and Bak1 in Rat Brain after Stroke." dans: CNS neuroscience & therapeutics, Vol. 21, Issue 6, pp. 496-503, (2016) (PubMed).

Détails sur BAK1

Antigène: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Autre désignation: Bcl-2-Like 7

Sujet: Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.Synonyms: Apoptosis regulator BAK, BAK, BAK1, BCL2L7, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, Bcl2-L-7, CDN1, cell death inhibitor 1

ID gène: 578

NCBI Accession: NP_001179

UniProt: Q16611

Pathways: Apoptose, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response