BAK1 anticorps (AA 22-211)

N° du produit ABIN3042342

Détail du produit anti-BAK1 anticorps

Antigène: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Épitope: AA 22-211

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BAK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: BCL2-antagonist/killer 1
Protein Name: Bcl-2 homologous antagonist/killer

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-BAK1 (ABIN3042342)

Zuo, Wu, Xu, Yang, Yan, Tan, Meng, Ying, Liu, Kang, Huang: "Minicircle-oriP-IFNγ: a novel targeted gene therapeutic system for EBV positive human nasopharyngeal carcinoma." dans: PLoS ONE, Vol. 6, Issue 5, pp. e19407, (2011) (PubMed).

Tong, Zheng, Wang, Liu, Qian: "BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells." dans: BMC cancer, Vol. 4, pp. 33, (2004) (PubMed).

Détails sur BAK1

Antigène: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Autre désignation: BAK1 (BAK1 Produits)

Synonymes: anticorps BAK, anticorps BAK-LIKE, anticorps BCL2L7, anticorps CDN1, anticorps Bak, anticorps N-BAK1, anticorps N-Bak, anticorps Bak1, anticorps bak, anticorps bak1, anticorps BCL2 antagonist/killer 1, anticorps BCL2-antagonist/killer 1, anticorps BCL2 antagonist/killer 1 L homeolog, anticorps BAK1, anticorps Bak1, anticorps bak1.L

Sujet: BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Synonyms: Apoptosis Regulator Bak antibody|BAK antibody|BAK like antibody|Bak NT antibody|BAK_HUMAN antibody|BAK1 antibody|Bcl 2 homologous antagonist/killer antibody|Bcl 2 like 7 protein antibody|Bcl-2 homologous antagonist/killer antibody|Bcl-2-like protein 7 antibody|BCL2 antagonist/killer 1 antibody|Bcl2 like 7 Protein antibody|Bcl2-L-7 antibody|BCL2L7 antibody|CDN1 antibody|Cell death inhibitor 1 antibody|MGC117255 antibody|MGC3887 antibody| NBak antibody|Pro apoptotic protein BAK antibody

ID gène: 578

UniProt: Q16611

Pathways: Apoptose, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response