VAV2 anticorps (Internal Region)

N° du produit ABIN374176

Détail du produit anti-VAV2 anticorps

Antigène: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp VAV2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Séquence: ETEAKYYRTL EDIEC

Specificité: This antobody is expected to recognise both reported isoforms of Human VAV2 (NP_001127870.1 and NP_003362.2).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Dog, and Pig.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from internal region of the protein sequence according to NP_001127870.1, NP_003362.2. Genename: VAV2

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-1.5 μg/mL. Approx 100 kDa band observed in 293 lysate (predicted MW of100 kDa according to NP_003362).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur VAV2

Antigène: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Autre désignation: VAV2 (VAV2 Produits)

Synonymes: anticorps VAV-2, anticorps 2810040F13Rik, anticorps AI847175, anticorps vav guanine nucleotide exchange factor 2, anticorps vav 2 oncogene, anticorps VAV2, anticorps Vav2

Sujet: VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.Synonyms: Guanine nucleotide exchange factor VAV2, VAV-2

ID gène: 7410

NCBI Accession: NP_001127870

UniProt: P52735

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway