ADAM17 anticorps (C-Term)

N° du produit ABIN374190

Cet anticorps anti-ADAM17 Polyclonal Chèvre (ABIN374190) détecte spécifiquement ADAM17 dans WB. L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour ADAM17 anticorps (C-Term) (ABIN374190)

Antigène: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivité: Humain, Rat, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ADAM17 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-ADAM17 anticorps

Épitope: C-Term

Séquence: LQRQNRVDSK ETEC

Specificité: This antibody detects CD156b / ADAM17 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_003174.3. Genename: ADAM17

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 100 kDa band observed in human testis lysates(calculated MW of 93.0 kDa according to NP_003174.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur ADAM17

Antigène: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Autre désignation: CD156b / ADAM17

Sujet: ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.Synonyms: CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme

ID gène: 6868

NCBI Accession: NP_003174

UniProt: P78536

Pathways: Signalisation Notch, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus