IRF5 anticorps (C-Term)

N° du produit ABIN374255

Détail du produit anti-IRF5 anticorps

Antigène: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IRF5 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Séquence: C-QGPWPMHPAG MQ

Specificité: This antibody is expected to recognize all four reported isoforms.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1. Genename: IRF5

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 57 kDa band observed in lysates of cell line A549. Intransfected HEK293 transiently expressing IRF5 a strong band of approx.67 kDa isobserved. This band is not observed in the non-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur IRF5

Antigène: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Autre désignation: IRF5 (IRF5 Produits)

Synonymes: anticorps IRF5, anticorps irf5, anticorps SLEB10, anticorps AW491843, anticorps mirf5, anticorps im:7155364, anticorps zgc:76986, anticorps interferon regulatory factor 5, anticorps interferon regulatory factor 5 L homeolog, anticorps IRF5, anticorps irf5, anticorps Irf5, anticorps irf5.L

Sujet: Interferon regulatory factors (IRFs) are transcription factors that possess a novel helix turn helix DNA binding motif and mediate virus and interferon (IFN) induced signaling pathways. The IRF5 protein is structurally related to IRF6. Like other IRFs, IRF5 and IRF6 contain 5 conserved tryptophan residues in the 115 amino acid N terminal DNA binding domain. IRF5 has a central IRF association domain (IAD) and, like IRF7 and IRF8, is induced by IFNA/IFNB stimulation. IRF5 has a type I IFN transactivation potential similar to that of IRF1. IRF3 and IRF5 form heterodimers and are both required for effective induction of IFNA gene transcription.Synonyms: IRF-5, Interferon regulatory factor 5

ID gène: 3663

NCBI Accession: NP_002191

UniProt: Q13568

Pathways: Signalisation TLR, Autophagy