NUP50 anticorps (C-Term)

N° du produit ABIN374317

Détail du produit anti-NUP50 anticorps

Antigène: NUP50 (Nucleoporin 50kDa (NUP50))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NUP50 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Séquence: C-ELHKILLEKK DA

Specificité: This antibody detects NUP50 at C-term. It is expected to recognise both human isoforms of this protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_009103.2, NP_705931.1. Genename: NUP50

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 60 kDa band seen in Jurkat lysate (predicted MW 54 kDaaccording to NP_009103).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur NUP50

Antigène: NUP50 (Nucleoporin 50kDa (NUP50))

Autre désignation: NUP50 (NUP50 Produits)

Synonymes: anticorps wu:fa56e03, anticorps wu:fb78a08, anticorps GB18194, anticorps npap60, anticorps npap60l, anticorps Nup50, anticorps Rtp60, anticorps 1700030K07Rik, anticorps AI413123, anticorps Npap60, anticorps NPAP60, anticorps NPAP60L, anticorps nucleoporin 50, anticorps nuclear pore complex protein Nup50, anticorps nucleoporin 50kDa S homeolog, anticorps nucleoporin 50kDa, anticorps nup50, anticorps LOC410864, anticorps NUP50, anticorps nup50.S, anticorps CpipJ_CPIJ012737, anticorps Nup50

Sujet: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa

ID gène: 10762

NCBI Accession: NP_009103

UniProt: Q9UKX7

Pathways: Tube Formation