KPNA2 anticorps (C-Term)

N° du produit ABIN374392

Détail du produit anti-KPNA2 anticorps

Antigène: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KPNA2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Séquence: C-QVQDGAPGTF NF

Specificité: This antibody detects Importin subunit alpha-2 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_002257.1 Genename: KPNA2

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.5 μg/mL. Approx 55-60 kDa band observed in Hela lysates (predictedsize of approx.50 kDa according to NP_002257.1). Immunofluoresence: in 4 % formaldahyde-fixed HeLa staining is mostly cytoplasmic, withsome nuclear staining and clear nuclear pore staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur KPNA2

Antigène: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Autre désignation: KPNA2 / Importin alpha-2 (KPNA2 Produits)

Synonymes: anticorps IPOA1, anticorps QIP2, anticorps RCH1, anticorps SRP1alpha, anticorps ima2, anticorps importin, anticorps 2410044B12Rik, anticorps PTAC58, anticorps Rch1, anticorps ipoa1, anticorps kpna2, anticorps qip2, anticorps rch1, anticorps srp1alpha, anticorps hm:zeh0389, anticorps hm:zeh0389r, anticorps zgc:113902, anticorps zgc:86945, anticorps karyopherin subunit alpha 2, anticorps importin subunit alpha-2, anticorps Importin subunit alpha-2, anticorps karyopherin (importin) alpha 2, anticorps karyopherin alpha-2 subunit like L homeolog, anticorps karyopherin alpha 2 (RAG cohort 1, importin alpha 1), anticorps KPNA2, anticorps EDI_246290, anticorps EDI_335040, anticorps ima2, anticorps Kpna2, anticorps kpna2.L, anticorps kpna2

Sujet: The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy independent docking of the protein to the nuclear envelope and the second is an energy dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.Synonyms: Karyopherin alpha-2, RAG cohort protein 1, RCH1, SRP1-alpha

ID gène: 3838

NCBI Accession: NP_002257

UniProt: P52292

Pathways: M Phase, Protein targeting to Nucleus