FBXO32 anticorps (Internal Region, Isoform 1)

N° du produit ABIN375094

Détail du produit anti-FBXO32 anticorps

Antigène: FBXO32 (F-Box Protein 32 (FBXO32))

Épitope: Internal Region, Isoform 1

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FBXO32 est non-conjugé

Application: Western Blotting (WB)

Specificité: This antibody is expected to recognize reported isoform 1 (FBXO32).

Réactivité croisée (Details): Species reactivity (expected):Rat, Bovine and Porcine.
Species reactivity (tested):Human, Mouse

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence: C-NSKTKTQYFHQEK from the internal region of F-box only protein 32 isoform 1 / FBXO32

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx.40 kDa band observed in Human and Mouse SkeletalMuscle lysates (calculated MW of 41.0 kDa according to Human NP_478136.1 and of41.0 kDa according to Mouse NP_080622.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur FBXO32

Antigène: FBXO32 (F-Box Protein 32 (FBXO32))

Autre désignation: FBXO32 (FBXO32 Produits)

Synonymes: anticorps MGC56479, anticorps zgc:56479, anticorps Fbx32, anticorps MAFbx, anticorps 4833442G10Rik, anticorps AI430017, anticorps ATROGIN1, anticorps Gm20361, anticorps Atrogin1, anticorps F-box protein 32, anticorps fbxo32, anticorps FBXO32, anticorps Fbxo32

Sujet: Fbx32 is an E3 ubiquitin ligase that initiates ATP dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. It is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. It is also thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Fbx32 interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD.Synonyms: Atrogin-1, F-box only protein 32, FBXO32, MAFbx, Muscle atrophy F-box protein

ID gène: 114907, 9606

UniProt: Q969P5