DAGLA anticorps (AA 1031-1042)

N° du produit ABIN396920

Détail du produit anti-DAGLA anticorps

Antigène: DAGLA (Diacylglycerol Lipase, alpha (DAGLA))

Épitope: AA 1031-1042

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DAGLA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human DAGLA.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Panda (92%) Mouse, Rat, Hamster, Bovine, Rabbit, Horse, Pig (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-PAKQDELVISAR from the C-terminus of human DAGLA (NP_006124.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Panda (92%), Mouse, Rat, Hamster, Bovine, Rabbit, Horse, Pig (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 125kD band observed in Human Liver lysates (calculated MW of 115kD according to NP_006124.1). Recommended concentration: 0.3-1 μg/mL. Some minor background is detected and is blocked by the immunizing peptide.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur DAGLA

Antigène: DAGLA (Diacylglycerol Lipase, alpha (DAGLA))

Autre désignation: DAGLA (DAGLA Produits)

Synonymes: anticorps C11orf11, anticorps DAGL(ALPHA), anticorps DAGLALPHA, anticorps NSDDR, anticorps Nsddr, anticorps diacylglycerol lipase alpha, anticorps diacylglycerol lipase, alpha, anticorps DAGLA, anticorps dagla, anticorps Dagla

Sujet: Name/Gene ID: DAGLA

Synonyms: DAGLA, DAGLALPHA, DGL-alpha, Diacylglycerol lipase, alpha, KIAA0659, C11orf11, DAGL(ALPHA), NSDDR

ID gène: 747

NCBI Accession: NP_006124

UniProt: Q9Y4D2