GST anticorps

N° du produit ABIN398843

Détail du produit anti-GST anticorps

Antigène: GST (Glutathione S Transferase (GST))

Reactivité: Schistosoma japonicum

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GST est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Goat Anti-GST-tag Polyclonal Antibody detects GST fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.

Purification: Immunoaffinity chromatography

Immunogène: Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.2-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-GST (ABIN398843)

Lichtenstein, Targan, Dubinsky, Rotter, Barken, Princen, Carroll, Brown, Stachelski, Chuang, Landers, Stempak, Singh, Silverberg: "Combination of genetic and quantitative serological immune markers are associated with complicated Crohn's disease behavior." dans: Inflammatory bowel diseases, Vol. 17, Issue 12, pp. 2488-96, (2011) (PubMed).

Détails sur GST

Antigène: GST (Glutathione S Transferase (GST))

Autre désignation: Glutathione-S-Transferase (GST) (GST Produits)

Synonymes: anticorps GST, anticorps GST 13-13, anticorps GST13, anticorps GST13-13, anticorps GSTK1-1, anticorps hGSTK1, anticorps 1500002K10Rik, anticorps Gst, anticorps Ptgds2, anticorps glutathione S-transferase kappa 1, anticorps microsomal glutathione S-transferase 1, anticorps hematopoietic prostaglandin D synthase, anticorps GSTK1, anticorps Mgst1, anticorps Hpgds

Sujet: Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be used in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.Goat Anti-GST-tag Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.