GPC1 anticorps (Internal Region)

N° du produit ABIN401058

Détail du produit anti-GPC1 anticorps

Antigène: GPC1 (Glypican 1 (GPC1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Chien, Macaque, Opossum

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPC1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody is specific for Glypican-1 protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Macaque, Canine, Cattle, Opossum.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: Synthetic peptide corresponding to an internal region of Human Glypican-1 protein.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1: 2,500 - 1: 3000. Western Blot: 1: 800 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 2.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Dilute only prior to immediate use. Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur GPC1

Antigène: GPC1 (Glypican 1 (GPC1))

Autre désignation: Glypican-1 / GPC1 (GPC1 Produits)

Synonymes: anticorps gpc1, anticorps zgc:63947, anticorps GPC1, anticorps Glypican-1, anticorps glypican, anticorps AI462976, anticorps HSPG M12, anticorps HSPGM12, anticorps glypican 1b, anticorps glypican 1, anticorps gpc1b, anticorps GPC1, anticorps gpc1, anticorps Gpc1

Sujet: Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17 % to 63 % identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.

ID gène: 2817

NCBI Accession: NP_002072

UniProt: P35052

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation