SPRY4 anticorps (AA 306-322)

N° du produit ABIN401284

Détail du produit anti-SPRY4 anticorps

Antigène: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Épitope: AA 306-322

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SPRY4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This affinity purified antibody is directed against Sprouty-4 protein.

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee (100 %).Bovine (94 %).Mouse (88 %).Rat and dog (82 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 100 - 1: 1,000 (Expect a band (maybe a doublet) approx. 35 kDa in sizecorresponding to Sprouty-4 in the appropriate cell lysate or extract). Immunohistochemistry on paraffin sections: 1 μg/mL to 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.93 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Détails sur SPRY4

Antigène: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Autre désignation: Sprouty Homolog 4 / SPRY4 (SPRY4 Produits)

Synonymes: anticorps SPRY4, anticorps LOC100218545, anticorps HH17, anticorps A030006O18Rik, anticorps sprouty4, anticorps sprouty RTK signaling antagonist 4, anticorps sprouty (Drosophila) homolog 4, anticorps sprouty homolog 4 (Drosophila), anticorps Spry4, anticorps SPRY4, anticorps spry4

Sujet: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.Synonyms: Sprouty 4, Spry-4

ID gène: 81848

NCBI Accession: NP_001120968

UniProt: Q9C004