L’anticorps anti-CHEK1 Polyclonal Lapin est utilisé pour la détection de CHEK1 dans des échantillons de Saccharomyces cerevisiae. Il a été validé pour WB et EIA.
ELISA: 1/2,000 - 1/10,000. Western Blot: 1/200 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.73 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Antigène
CHEK1
(Checkpoint Kinase 1 (CHEK1))
Autre désignation
CHK1
Sujet
CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localized within the nucleus.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1