p130 anticorps
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- Antigène Voir toutes p130 (RBL2) Anticorps
- p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
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Reactivité
- Humain, Rat, Boeuf (Vache), Chien, Cheval, Chimpanzé, Orang-Utan, Opossum
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp p130 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Specificité
- This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues.
- Réactivité croisée (Details)
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Species reactivity (expected):Chimpanzee, Orangutan, Horse, CAnine, Bovine, Rat, Opossum.
Species reactivity (tested):Human. - Purification
- Affinity chromatography
- Immunogène
- Synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residue of cysteine was added to facilitate coupling
- Isotype
- IgG
- Top Product
- Discover our top product RBL2 Anticorps primaire
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- Indications d'application
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ELISA: 1/75,000 - 1/100,000. Western Blot: 1/500 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.39 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.09 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
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- Antigène
- p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
- Autre désignation
- RBL2 / p130 (RBL2 Produits)
- Synonymes
- anticorps RBL2, anticorps RBR2, anticorps RRB2a, anticorps PRB2, anticorps RBR-2, anticorps Rb2, anticorps p130, anticorps P130, anticorps retinoblastoma-like 2 (p130), anticorps RB transcriptional corepressor like 2, anticorps related to retinoblastoma 2, anticorps RBL2, anticorps LOC541652, anticorps Rbl2
- Sujet
- Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play wellrecognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.Synonyms: 130 kDa retinoblastoma-associated protein, RB2, RBR-2, Retinoblastoma-like protein 2, Retinoblastoma-related protein 2, p130, pRb2
- ID gène
- 5934
- NCBI Accession
- NP_005602
- UniProt
- Q08999
- Pathways
- Cycle Cellulaire, Mitotic G1-G1/S Phases
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