ATM anticorps (pSer1981)

N° du produit ABIN401482

Détail du produit anti-ATM anticorps

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Épitope: pSer1981

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp ATM est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against ATM.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH using maleimide

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur ATM

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Autre désignation: ATM (ATM Produits)

Synonymes: anticorps ATM, anticorps Atm, anticorps CG6535, anticorps Dmel\\CG6535, anticorps Tefu, anticorps atm, anticorps atm/tefu, anticorps dATM, anticorps tef, anticorps Xatm, anticorps at1, anticorps atdc, anticorps tel1, anticorps telo1, anticorps AT1, anticorps ATA, anticorps ATC, anticorps ATD, anticorps ATDC, anticorps ATE, anticorps TEL1, anticorps TELO1, anticorps AI256621, anticorps C030026E19Rik, anticorps telomere fusion, anticorps ATM serine/threonine kinase L homeolog, anticorps ATM serine/threonine kinase, anticorps ataxia telangiectasia mutated, anticorps ataxia telangiectasia mutated (atm), anticorps serine/threonine-protein kinase ATM, anticorps tefu, anticorps atm.L, anticorps atm, anticorps ATM, anticorps EDI_100660, anticorps CpipJ_CPIJ001772, anticorps BDBG_08252, anticorps PAAG_02532, anticorps MCYG_05088, anticorps VDBG_06833, anticorps ACLA_015700, anticorps LOC5565620, anticorps MGYG_07634, anticorps PGTG_14279, anticorps Atm

Sujet: ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation (IR) for instance) is central to understanding its function. ATM protein is present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. This antibody is similar to the rabbit host antibody discussed by Bakkenist, C. J. & Kastan, M. B. in Nature 421, 499-506 (2003).Synonyms: A-T mutated, ATDC, Ataxia telangiectasia mutated, Serine-protein kinase ATM, TEL1, TELO1

ID gène: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus