Cyclin B1 anticorps (Ser147)
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- Antigène Voir toutes Cyclin B1 (CCNB1) Anticorps
- Cyclin B1 (CCNB1)
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Épitope
- Ser147
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Cyclin B1 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- Cyclin B1 Antibody detects endogenous levels of total Cyclin B1 protein.
- Purification
- Affinity chromatography
- Immunogène
- Synthesized non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V)
- Top Product
- Discover our top product CCNB1 Anticorps primaire
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- Indications d'application
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Immunohistochemistry: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
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- Antigène
- Cyclin B1 (CCNB1)
- Autre désignation
- Cyclin B1 (CCNB1 Produits)
- Synonymes
- anticorps ccnb, anticorps cycb, anticorps cb267, anticorps cycb1, anticorps wu:fa19g04, anticorps wu:fb16d01, anticorps wu:fb16e07, anticorps wu:fi21c01, anticorps ccnb1, anticorps MGC53596, anticorps CCNB, anticorps Ccnb1-rs1, anticorps Ccnb1-rs13, anticorps CycB1, anticorps Cycb-4, anticorps Cycb-5, anticorps Cycb1-rs1, anticorps cyclin B1, anticorps cyclin B1 S homeolog, anticorps ccnb1, anticorps ccnb1.2, anticorps ccnb1.S, anticorps CCNB1, anticorps Ccnb1, anticorps ccnb1.2.S
- Sujet
- In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.Synonyms: CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1
- ID gène
- 891
- NCBI Accession
- NP_114172
- UniProt
- P14635
- Pathways
- Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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