p53 anticorps (AA 20-31)

N° du produit ABIN4219254

Cet anticorps anti-p53 est un anticorps Souris Monoclonal détectant p53 dans WB, ELISA, IP, IHC (fp) et IHC (fro). Adapté pour Humain. Ce Primary Antibody a été cité dans 9+ publications.

Aperçu rapide pour p53 anticorps (AA 20-31) (ABIN4219254)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Bp53-11

Détail du produit anti-p53 anticorps

Épitope: AA 20-31

Specificité: P53

Réactivité croisée: Humain

Purification: Protein A affinity column

Immunogène: Recombinant human p53 (transcription domain within the NH2 terminus)

Isotype: IgG2a

Information d'application

Indications d'application: IHC: 1:500, when using the APAAP method

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: reconstitue with 1 mL deionized water

Concentration: 0.025 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: 2-8°C, extended storage at -20°C, avoid repeated freeze-thaw cycles

Références pour anticorps anti-p53 (ABIN4219254)

Zarka, Han, Edelson, Rosenblum: "Expression of cadherins, p53, and BCL2 in small cell carcinomas of the cervix: potential tumor suppressor role for N-cadherin." dans: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, Vol. 13, Issue 2, pp. 240-3, (2003) (PubMed).

Ohtaké, Cheng, Ida, Suzuki, Ohshiro, Zhang, Saku: "Precancerous foci in pleomorphic adenoma of the salivary gland: recognition of focal carcinoma and atypical tumor cells by P53 immunohistochemistry." dans: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 31, Issue 10, pp. 590-7, (2002) (PubMed).

Homann, Nees, Conradt, Dietz, Weidauer, Maier, Bosch: "Overexpression of p53 in tumor-distant epithelia of head and neck cancer patients is associated with an increased incidence of second primary carcinoma." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 2, pp. 290-6, (2001) (PubMed).

Andl, Kahn, Pfuhl, Nicola, Erber, Conradt, Klein, Helbig, Dietz, Weidauer, Bosch: "Etiological involvement of oncogenic human papillomavirus in tonsillar squamous cell carcinomas lacking retinoblastoma cell cycle control." dans: Cancer research, Vol. 58, Issue 1, pp. 5-13, (1998) (PubMed).

Petersen, Song, Hügle-Dörr, Oldenburg, Bautz: "Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries." dans: Molecular & general genetics : MGG, Vol. 249, Issue 4, pp. 425-31, (1996) (PubMed).

Stephen, Helminen, Lane: "Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions." dans: Journal of molecular biology, Vol. 248, Issue 1, pp. 58-78, (1995) (PubMed).

Fisher, Gillett, Vojtĕsek, Barnes, Millis: "Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation." dans: British journal of cancer, Vol. 69, Issue 1, pp. 26-31, (1994) (PubMed).

Bártek, Bártková, Lukás, Stasková, Vojt?sek, Lane: "Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies." dans: The Journal of pathology, Vol. 169, Issue 1, pp. 27-34, (1993) (PubMed).

Bártek, Bártková, Vojtĕsek, Stasková, Rejthar, Kovarík, Lane: "Patterns of expression of the p53 tumour suppressor in human breast tissues and tumours in situ and in vitro." dans: International journal of cancer, Vol. 46, Issue 5, pp. 839-44, (1990) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: P53

Sujet: Excellent marker for wild-type and mutant forms of human p53 antigen. The antibody stains positively approx. 60 % of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors, T-cell Leuk

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg