Reactive to serine133 phosphorylated Human CREB peptide fragment, and reactive to KLH or BSA conjugated Serine133 phosphorylated Human CREB peptide fragment. Not reactive to un-conjugated KLH or BSA.
Réactivité croisée (Details)
Species reactivity (tested):Human.
Attributs du produit
Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsiveelement-binding protein 1
Purification
Affinity Chromatography on Protein G.
Immunogène
Phosphorylated peptide fragment of CREB (pSer133), conjugated with KLH
CREB1
Reactivité: Humain, Souris, Rat
WB, IHC, IF, FACS, IP, ICC
Hôte: Lapin
Monoclonal
unconjugated
Indications d'application
Indirect ELISA: The suggested antigen coating quantity is 1 μg/mL of peptide/or peptideconjugating in pH 9.5 Carbonate buffer, 100 μL/well. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
Buffer
0.01 M PBS, pH 7.0 without preservatives.
Agent conservateur
Without preservative
Stock
-20 °C
Stockage commentaire
Store the antibody at -20 °C. After reconstitution, aliquot and store at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Date de péremption
12 months
Antigène
CREB1
(cAMP Responsive Element Binding Protein 1 (CREB1))
anticorps creb, anticorps CREB1, anticorps Creb1, anticorps CREB, anticorps 2310001E10Rik, anticorps 3526402H21Rik, anticorps AV083133, anticorps Creb, anticorps Creb-1, anticorps creb1, anticorps zgc:55598, anticorps cAMP responsive element binding protein 1, anticorps cAMP responsive element binding protein, anticorps cAMP responsive element binding protein 1 S homeolog, anticorps cAMP responsive element binding protein 1a, anticorps CREB1, anticorps creb1, anticorps CREB, anticorps Creb1, anticorps creb1.S, anticorps creb1a
Sujet
Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1