DLC1 anticorps (Isoform 1, Isoform 3, N-Term)

N° du produit ABIN452680

Détail du produit anti-DLC1 anticorps

Antigène: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Épitope: Isoform 1, Isoform 3, N-Term

Reactivité: Humain, Souris, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DLC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: SVAIRKRSWE EHC

Specificité: This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3) but NOT isoform 2 (NP_006085.2).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the N Terminus of the protein sequence according to NP_872584.2, NP_079043.3. Genename: DLC1

Information d'application

Indications d'application: Peptide ELISA: Antibody Detection Limit Dilution: 1/2,000. Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Liver andLung lysates as well as in different epithelial lung cell lines after 0.3 μg/mL antibodystaining. Please note that currently we cannot find an explanation in the literature for theband we observe given the calculated size of 171 kDa according to NP_872584.1 and54.8 kDa according to NP_079043.2 (isotope 3). The 28 kDa band was successfully blockedby incubation with the immunizing peptide. Immunohistochemistry: 5 μg/mL. In paraffin embedded Human Adrenal Gland (Medulla)shows strong reticulate staining in the cytoplasm of medular cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur DLC1

Antigène: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Autre désignation: ARHGAP7 / DLC1 (DLC1 Produits)

Synonymes: anticorps ARHGAP7, anticorps HP, anticorps STARD12, anticorps p122-RhoGAP, anticorps rhogap7, anticorps DLC-1, anticorps StARD12, anticorps Dlc-1, anticorps A730069N07Rik, anticorps Arhgap7, anticorps dlc-1, anticorps RhoGAP, anticorps Dlc1, anticorps DLC1 Rho GTPase activating protein, anticorps DLC1 Rho GTPase activating protein S homeolog, anticorps rho GTPase-activating protein 7, anticorps deleted in liver cancer 1, anticorps DLC1, anticorps dlc1.S, anticorps dlc1, anticorps LOC100456847, anticorps Dlc1, anticorps LOC100729904

Sujet: DLC1 functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization. DLC1 is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.Synonyms: Deleted in liver cancer 1 protein, HP protein, KIAA1723, Rho GTPase-activating protein 7, Rho-type GTPase-activating protein 7, STARD12, START domain-containing protein 12, StAR-related lipid transfer protein 12

ID gène: 10395

NCBI Accession: NP_006085

UniProt: Q96QB1

Pathways: Tube Formation, Positive Regulation of Endopeptidase Activity