SERPING1 anticorps

N° du produit ABIN458102

Détail du produit anti-SERPING1 anticorps

Antigène: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SERPING1 est non-conjugé

Application: Immunoelectrophoresis (IEP), Immunodiffusion (ID), Radial Immunodiffusion (RID)

Specificité: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Precipitating polyclonal rabbit antiserum to human C1 esterase inhibitor

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: C1 esterase inhibitor is a single chain acid glycoprotein with a molecular weight of 110,000. The protein has a high carbohydrate content. In immunoelectrophoresis it ha an alpha mobility. The concentration in normal serum ranges from 150 to 350 µ g/ml. Reduced levels are observed in hereditary angioedema. It regulates the activation of complement factor C1 as well as the activity of activated C1 by coupling with the active catalytic site at the light chains of C1r and C1s. Moreover it also inhibits, kallikrein, Factor XI and Factor XII. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C1 esterase inhibitor in human plasma or other body fluids or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. In immunoelectrophoresis use 2 µ l or equivalent against 120 µ l antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µ l in a 3 mm diameter center well and 2 µ l serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:16 when tested against pooled normal human plasma in agar-block immuno-diffusion tit

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Agent conservateur: Without preservative

Stock: 4 °C

Détails sur SERPING1

Antigène: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Autre désignation: C1 Esterase Inhibitor (SERPING1 Produits)

Synonymes: anticorps fb64f02, anticorps im:7159527, anticorps si:dkeyp-110g5.2, anticorps wu:fb60c09, anticorps wu:fb62a08, anticorps wu:fb64f02, anticorps LOC100136072, anticorps C1-INH, anticorps C1INH, anticorps C1nh, anticorps C1IN, anticorps C1NH, anticorps HAE1, anticorps HAE2, anticorps serpin peptidase inhibitor, clade G (C1 inhibitor), member 1, anticorps C1 inhibitor, anticorps serpin family G member 1, anticorps serine (or cysteine) peptidase inhibitor, clade G, member 1, anticorps serping1, anticorps LOC100136072, anticorps SERPING1, anticorps Serping1

Sujet: The reactivity of the antiserum is restricted to C1 esterase inhibitor. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C1 esterase inhibitor. No reaction is obtained with any other plasma protein component or serum

Pathways: Système du Complément