Oncostatin M anticorps

N° du produit ABIN465829

Détail du produit anti-Oncostatin M anticorps

Antigène: Oncostatin M (OSM)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Oncostatin M est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificité: Human Oncostatin-M

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human Oncostatin-M.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 - 0.18 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hOncostatin M (10 ng/mL), a concentration of 0.10-0.175 μg/mL of this antibody is required. ELISA: To detect hOncostatin M by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Oncostatin M (LS-C104706) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hOncostatin M. Western Blot: To detect hOncostatin M by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin M is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur Oncostatin M

Antigène: Oncostatin M (OSM)

Autre désignation: OSM / Oncostatin M (OSM Produits)

Synonymes: anticorps OSM, anticorps OncoM, anticorps oncostatin M, anticorps OSM, anticorps Osm

Sujet: Name/Gene ID: OSM

Synonyms: OSM, Oncostatin-M, Oncostatin M

ID gène: 5008

UniProt: P13725

Pathways: Signalistation JAK/STAT, Negative Regulation of Hormone Secretion