MLL4 anticorps (C-Term)

N° du produit ABIN488001

Détail du produit anti-MLL4 anticorps

Antigène: MLL4 (Histone-Lysine N-Methyltransferase MLL4 (MLL4))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MLL4 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody reacts to MLL4.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Peptide with sequence CNCGAKRCRRFLN, from the C Terminus of the protein sequence according to NP_055542.

Information d'application

Indications d'application: Peptide ELISA: 1/128000. Western blot: Preliminary experiments gave no signal but low background in Human Heartlysates at up to 1 μg/mL. However this used our routine western blotting protocol which wewould not expect to reliably detect proteins as large as the predicted size of approx.312KDa according to NP_055542. Immunohistochemistry on paraffin sections: Human Testis shows cytoplasm staining ofspermatids. Recommended concentration: 5 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur MLL4

Antigène: MLL4 (Histone-Lysine N-Methyltransferase MLL4 (MLL4))

Autre désignation: MLL4 (MLL4 Produits)

Synonymes: anticorps HRX2, anticorps MLL1B, anticorps MLL2, anticorps MLL4, anticorps TRX2, anticorps WBP7, anticorps 2610014H22Rik, anticorps Mll2, anticorps Wbp7, anticorps mKIAA0304, anticorps ALR, anticorps BC032281, anticorps BC058659, anticorps C430014K11Rik, anticorps KMT2B, anticorps Mll4, anticorps RGD1308331, anticorps lysine methyltransferase 2B, anticorps lysine (K)-specific methyltransferase 2B, anticorps lysine (K)-specific methyltransferase 2D, anticorps KMT2B, anticorps Kmt2b, anticorps Kmt2d

Sujet: KMT2D / MLL4 contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This protein is ubiquitously expressed in adult tissues where it possibly acts as a transcriptional regulatory factor. It is also expressed in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced gene transcripts encoding distinct isoforms have been reported, however, the full length nature of the shorter transcript is not known.Synonyms: HRX2, Histone-lysine N-methyltransferase MLL4, KIAA0304, KMT2D, Lysine N-methyltransferase 2D, MLL2, Myeloid/lymphoid or mixed-lineage leukemia protein 4, TRX2, Trithorax homolog 2, WBP7, WW domain-binding protein 7

ID gène: 9757

NCBI Accession: NP_055542

UniProt: Q9UMN6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, L'effet Warburg