ERK2 anticorps (N-Term) (Biotin)

N° du produit ABIN492352

Cet anticorps Souris Monoclonal détecte spécifiquement ERK2 dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour ERK2 anticorps (N-Term) (Biotin) (ABIN492352)

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERK2 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 6H3

Détail du produit anti-ERK2 anticorps (Biotin)

Épitope: N-Term

Specificité: This antibody specifically recognizes the N-terminus of MAP kinase 2 (ERK2).

Attributs du produit: Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Peptide conjugated to KLHEpitope: N-terminus

Isotype: IgG1

Information d'application

Indications d'application: ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % Sodium Azide/PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Aliquote and freeze in liquid nitrogenAntibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.

Date de péremption: 12 months

Détails sur ERK2

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Autre désignation: ERK2

Sujet: Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK

ID gène: 5594

UniProt: P28482

Pathways: Signalisation MAPK, Signalisation RTK, Apoptose, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins